Part:BBa_K1507020

Skin whitening substance generator (PelB signal sequence + PKEK)

In E.coli Type II secretion system, the signal sequence(In here is PelB) can bring the target protein to the inner-membrane， and transfer the target protein to the outer space. There are many candidates sequence for guiding or protein product, so we choose three of them(All of them have been studied for a long period of time by other laboratory), to make sure the signal sequence is suit for the transportation of our target proteins. PKEK, comprise of Pro-Lys-Glu-Lys, is a tetraoligopeptide that has the strong effect of whitening our skin. By down-regulating the mRNA expression of interleukin (IL)-6, IL-8 and TNF-α and tyrosinase, it can prevent the formation of eumelanin. Another advantage why we choose PKEK is it can easily penetrate the skin barrier and have its maximum

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 54
  
• 1000
  COMPATIBLE WITH RFC[1000]